iLite® ADCC Target mTNF-alpha (-) Assay Ready Cells

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iLite® ADCC Target mTNF-alpha (-) Assay Ready Cells are based on a human embryonic kidney cell line, HEK293 (ATCC# CRL-1573), and have been genetically engineered and optimized to deplete all expression of the surface protein mTNF-alpha. The cells are for use as internal controls together with iLite® ADCC Effector (V) Assay Ready Cells and iLite® ADCC Target mTNF-alpha (+) Assay Ready Cells when measuring the ADCC activity of anti-TNF-alpha antibodies.

Product code
BM5014
Calculation
Quantitative
Antigen
None
Detection system
Luminescence
Availability
Research Use Only (RUO)

The iLite® anti- ADCC Target mTNF-alpha (-) Assay Ready Cells can be used as an internal control together with iLite® ADCC Effector (V) and iLite® ADCC Target mTNF-alpha (-) Assay Ready Cells when quantificatying ADCC activity. Please see:

  • Quantification of anti-mTNF-alpha ADCC activity (LABEL-DOC0402)

Antibody-dependent cell-mediated cytotoxicity (ADCC) is a mechanism whereby pathogenic cells are lysed by lymphocytes, most often Natural Killer (NK) cells. The mechanism involves binding of antibodies to surface antigens on the pathogen. Crosslinking of these antibodies to NK cells through the binding of the Fc-portion to Fc receptors on the NK cells leads to activation of the NK cell and formation of an immune synapse with the pathogenic cell. The NK cell releases cytotoxic granules containing granzymes and perforin into the synapse, leading to apoptosis of the targeted cell (1). 

>250 µL of iLite® Assay Ready Cells suspended in cryoprotective medium from Gibco (cat no 12648-010).