iLite® IL-2 Assay Ready Cells

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iLite® IL-2 Assay Ready Cells are avian DT-40 cells (ATCC# CRL-2111) which have been genetically engineered and optimized to be responsive to IL-2, resulting in a proportional expression of Firefly Luciferase. Normalization of cell counts, and serum matrix effects is obtained by a second reporter gene, a Renilla Luciferase reporter gene construct, under control of a constitutive promotor.

Product code
BM4002
Format
Assay Ready Cells
Calculation
Quantitative
Antigen
IL-2
Detection system
Luminescence
Availability
Research use only

The iLite® IL-2 Assay Ready Cells can be used for the quantification of IL-2 activity, or neutralizing antibody response against IL-2 in test samples, including human serum.

Application Notes for the following assays are available:

  • Quantification of functional IL-2 
  • Quantification of IL-2 inhibitor 

 

IL-2 is an interleukin and part of the bodys natural response to microbial infection and a tool in the immune system discrimination between foreign ("non-self") and "self". It signals through the IL-2 receptor, a complex consisting of three chains, termed alpha, beta, and gamma.

IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily as a stimulant of T- and B-cell growth and maturation. IL-2 activates and stimulates the growth of immune cells, most importantly T-Cells, but also Natural Killer Cells (NK Cells), both can clear cancer cells directly. Anti-tumour effects of IL-2 appear to be mediated by its effects on NK Cells, Lymphokine-activated Killer Cells and other cytotoxic cells (1,2).

>250 µL of Assay Ready Cells suspended in RPMI 1640 with 20% heat inactivated fetal bovine serum (FBS), mixed 1:1 with cryoprotective medium from Lonza (Cat. No 12-132A).

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