Complement TCC

Add to my product list
Complement TCC is an excellent marker for complement studies in basic and translational research, clinical study of treatment, safety studies of medical device and drugs.

The Svar Complement TCC is a flexible and easy to use enzyme immunoassay for determination of the terminal complement complex (TCC, also known as sC5b-9) in human plasma.

The complement system plays an essential role in acute and chronic inflammation, in immune responses to infections and in autoimmune diseases. As TCC is a product of the terminal pathway and can be generated from all three complement pathways, TCC is used  in all situations where determination of complement activity is wanted.

TCC is also well suited for studies of complement activation in drug development and medical devices, i.e. biological safety testing and part of the recommended tests for assessing complement activation according to ISO standard 10993-4 for hemocompatibility testing.

Product code
COMPL TCC RUO
Format
ELISA
Tests
Break apart microtitration strips (12x8) 96 wells
Calculation
Semi-Quantitative
Incubation time
60+30+30 min
Detection system
450 nm
Availability
For Research Use Only. Not for use in diagnostic procedures.

The Complement TCC is an enzyme immunoassay intended for the semi-quantitative determination of terminal complement complex (TCC also known as sC5b-9) in human EDTA plasma.

The product is intended for professional use. The result of the assay can be used for measurement of complement activation in human plasma samples, but shall not be used for clinical diagnosis or patient management. FOR RESEARCH USE ONLY.

The assay is a colorimetric sandwich ELISA.

Samples are diluted in assay diluent and 100µL diluted sample is transferred to the microtiter wells and incubated at room temperature for 60 minutes. During this first incubation TCC in the sample is captured by the anti-TCC monoclonal antibody, pre-coated on the surface of the microtiter wells.

After washing to remove unbound material, a second horseradish peroxidase (HRP) labelled monoclonal antibody is added to detect the TCC bound to the well. After incubation for 30 minutes the wells are washed again and a substrate is added and incubated. The color development is stopped after 30 minutes and the color is measured in a spectrophotometer. The color is directly proportional to the amount of TCC bound to the well.

The amount of TCC is determined by comparison to the color development of the calibrator samples.

  • One frame with microtiter wells (12x8) coated with anti-TCC monoclonal antibody, sealed in a foil pack with a dry pack.
  • 1.5mL Low control (LC). Ready to use.
  • 1.5mL High control (HC). Ready to use.
  • 2 x 30 mL Diluent (Dil). Ready to use.
  • 15 mL Conjugate containing HRP-labelled antibodies to TCC. Ready to use.
  • 15 mL Substrate TMB. Ready to use.
  • 15 mL Stop solution (0.5M H2SO4). Ready to use.
  • 30 mL Wash solution, 30x concentrated.
  • 6 x 1.5mL vials with calibrators containing human purified TCC, 0 ng/mL, 10ng/mL, 50 ng/mL, 100ng/mL, 200ng/mL and 400ng/mL. Ready to use.

Please note:
All reagents in the kit are ready to use except the wash solution. The reagents should be stored at 2- 8°C. Components from different lots shall not be mixed.

Materials or equipment required but not provided

  • Microplate reader with filter 450nm and 620nm.
  • Precision pipettes with disposable tips.
  • Washer for strips, absorbent tissue, tubes and a timer.
  • Dilution plate.